Notre Dame will be without a key defensive player when the Irish (6-2) play host to Navy (4-3) on Saturday.
Coach Brian Kelly said Tuesday that linebacker Ishaq Williams will not play against the Midshipmen.
Williams made his first start this season in last weekend's win over Air Force, but the junior injured his knee early in the first quarter and had to leave the game against the Falcons.
Kelly had said Sunday that Williams was questionable to play Saturday.
Notre Dame is still waiting to see if starting nose guard Louis Nix III can play this week. The senior didn't play against Air Force because of tendinitis in his knee.
Kelly said Nix is questionable to play.
"He's progressing well," Kelly said. "He worked out yesterday, but we won't know of his status. Depending on how he moves and what he can do. We're not prepared to play anybody that can't get in there and practice and do the things necessary to prepare for the triple option."
Notre Dame starting left guard Chris Watt and defensive end Sheldon Day remained probably for the game against Navy. Watt injured his knee and Day re-tweaked his ankle against Air Force.
"Watt looked good today, we're going to move him around," Kelly said. "Day is probably. We've got an MRI on that ankle. We wanted to rule out any further high ankle damage to the ligaments, and it was a bone bruise. So it was totally different. So we feel pretty good about that. He'll practice today."
Kelly said right tackle Ronnie Stanley, who had to come out against Air Force because of an injury, and receiver DaVaris Daniels, who suffered a hip flexor against the Falcons, will practice this week. Both players will likely play against Navy.
Safety Elijah Shumate, who missed the last two games with a hamstring injury, could be available this week. Kelly said Shumate is moving around well and is expected to practice Tuesday.
"What his role will be, we'll determine that as we work our way through the week," Kelly said.
Kelly also said defensive end Chase Hounshell, who suffered a right shoulder injury in spring practice, has started drill work in pads, but the junior likely will not play this year.